Genome mining of fungal secondary metabolite biosynthetic gene clusters containing a gene encoding HRPKS-TE megasynthase. (A) KU42 cluster mined from P. strigosozonata and the production of 1 and 2 in yeast. (B) KU43 cluster mined from H. pinastri and the production of 3 in yeast. HP, hypothetical protein; PAP2, type 2 phosphatidic acid phosphatase; MFS, major facilitator superfamily; C-MT, carboxyl methyltransferase. Shown are the traces of extracted-ion chromatogram. In control traces from strains transformed with vector only, these compounds were not found.