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. Author manuscript; available in PMC: 2020 May 22.
Published in final edited form as: J Am Chem Soc. 2019 May 10;141(20):8198–8206. doi: 10.1021/jacs.9b01083

Figure 3.

Figure 3

Characterization of KU42 HRPKS-TE in vivo and in vitro. Shown are the extracted-ion chromatogram. (A) Expression of HRPKS-TE and C2040A mutant in yeast and the structures of the compounds produced; (B) LC-MS analysis of the in vitro assays of purified HRPKS-TE in the presence of malonyl-CoA, NADPH and l-cysteine or l-homocysteine.