Figure 3.

Stimulation with anti-IFN-γ autoAbs decreases IFN-γ-induced generation of nitrite and hydrogen peroxide in differentiated macrophages. After treatment with anti-IFN-γ autoAb-positive patient serum (PS) or anti-IFN-γ autoAb-negative healthy serum (HS), (a) Western blot analysis, (b) Griess reaction, and (c) CM-H₂DCFDA staining showed the biosynthesis of iNOS, the biosynthesis of NO, and the generation of hydrogen peroxide in IFN-γ-stimulated PMA-differentiated macrophages, respectively. The quantitative data are shown as the mean ± SD from three independent experiments. For DCFDA detection, the stained cells were analyzed using a flow cytometer and the data are shown as the mean fluorescence intensity (MFI). ^∗∗∗p < 0.001 compared with the control group. ^###p < 0.001 compared with the HS group.