Table 2.

PCR-RFLP: primer sequences, PCR conditions, restriction enzymes, and fragment lengths.

Polymorphism	Primer sequences	PCR protocol	Gel electrophoresis results
GSTA1∗C-69T	F, 5′-GCATCAGCTTGCCCTTCA -3′ R, 5′-AAACGCTGTCACCGTCCTG-3′	Denature: 94°C for 3 min Followed by 94°C for 30 s Annealing: 56°C for 30 s Extension: 72°C for 30 s #cycles: 30 Final extension: 72°C for 10 min	Eam1104I incubation at 37°C overnight GSTA1∗CC: 400 bp GSTA1∗CT: 400 bp, 308 bp, and 92 bp GSTA1∗TT: 308 bp and 92 bp
GSTP1∗Ile105Val	F, 5′-ACCCCAGGGCTCTATGGGAA-3′ R, 5′-TGAGGGCACAAGAAGCCCCT-3′	Denature: 95°C for 10 min Followed by 94°C for 30 s Annealing: 59°C for 30 s Extension: 72°C for 30 s #cycles: 29 Final extension: 72°C for 10 min	Alw26I incubation at 37°C overnight GSTP1∗Ile/Ile: 176 bp GSTP1∗Ile/Val: 176 bp, 91 bp, and 85 bp GSTP1∗Val/Val: 91 bp and 85 bp
GSTM1	F, 5′-GAACTCCCTGAAAAGCTAAAGC-3′ R, 5′-GTTGGGCTCAAATATACGGTGG-3′	Multiplex PCR: Denature: 94°C for 3 min Followed by 94°C for 30 s Annealing: 59°C for 30 s Extension: 72°C for 45 s #cycles: 30 Final extension: 72°C for 4 min	GSTM1∗active: 215 bp band GSTM1∗null: absent band
GSTT1	F, 5′-TTCCTTACTGGTCCTCACATCTC-3′ R, 5′-TCACGGGATCATGGCCAGCA-3′		GSTT1∗active: 480 bp band GSTT1∗null: absent band
CYP1A1	F, 5′-GAACTGCCACTT CAGCTGTCT-3′ R, 5′-CAGCTGCATTTG GAAGTGCTC-3′		Successful PCR reaction: 312 bp band Unsuccessful PCR reaction: absent band