Table 1.

Clinicopathological data and association with SOX9 in all patients included in the study.

	All cases		SOX9 negative		SOX9 positive		P
	n	%	n	%	n	%
Patients	333	100	58	17.4	275	82.6
Age
Media ± SD	67.6±11.9		67.3±9.6		67.7±12.4		0.74
Range	32-95		46-83		32-95
Gender
Female	145	43.5	23	15.9	122	84.1	0.56
Male	188	56.5	35	18.6	153	81.4
Laurén classification
Intestinal	156	46.9	26	16.7	130	83.3	0.85
Diffuse	44	13.2	8	18.2	36	81.8
Mixed	87	26.1	17	19.5	70	80.5
Unclassified	46	13.8
Growth pattern
Expansive	61	18.3	13	21.3	48	78.7	0.34
Infiltrative	258	77.5	41	15.9	217	84.1
Unclassified	14	4.2
WHO classification
Tubular	146	43.8	25	17.1	121	82.9	0.99
Papillary	1	0.3	0	0.0	1	100.0
Poorly cohesive	37	11.1	7	18.9	30	81.1
Mucinous	7	2.1	1	14.3	6	85.7
Other variants	142	42.7	25	17.6	117	82.4
TNM
I	112	33.7	17	15.2	95	84.8	0.04∗
II	87	26.1	21	24.1	66	75.9
III	69	20.7	15	21.7	54	78.3
IV	65	19.5	5	7.7	60	92.3
Resection margins
R0	298	89.5	53	17.8	245	82.2	0.48
R1/R2	34	10.2	4	11.8	30	88.2
ND	1	0.3
Vascular invasion
No	140	42.0	23	16.4	117	83.6	0.66
Yes	190	57.1	35	18.4	155	81.6
ND	3	0.9
Perineural invasion
No	173	52.0	33	19.1	140	80.9	0.47
Yes	159	47.7	25	15.7	134	84.3
ND	1	0.3
β-Catenin nuclear expression
No	219	65.8	43	19.6	176	80.4	0.07
Yes	91	27.3	10	11.0	81	89.0
ND	23	6.9
Chemotherapy
Yes	124	37.2	27	46.6	97	35.8	0.14
No	205	61.6	31	53.4	174	64.2
ND	4	1.2

Notes. P values (statistical significance threshold < 0.05) were obtained using Student's t-test for the continuous variable, Fisher's exact test (2-sided), and chi-square (χ ²) test for categorical variables. ^∗Comparisons with P < 0.05. ND = not determined; SD = standard deviation.