Figure 3.

Effect of CuB on oxidative stress in yeast. (a) Effect of CuB on yeast growth with 5 μL yeast culture and 1/10, 1/100, and 1/1000 dilutions under oxidative conditions simulated with 9 mM H₂O₂. (b) Effect of CuB on the survival rate of yeast under oxidative conditions simulated with 5 mM H₂O₂. ∗∗ and ∗∗∗ represent significant differences compared to the control group (p < 0.01 and p < 0.001, respectively). (c, d, e, f) Effect of CuB on ROS level, MDA accumulation, T-Sod, and Sod1 enzyme activity at 24 or 48 h. ∗, ∗∗, ∗∗∗ and #, ##, ### indicate significant differences from the corresponding control (p < 0.05, p < 0.01, and p < 0.001, respectively).