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. 2019 Jun 20;7:e7031. doi: 10.7717/peerj.7031

Table 1. Primers for amplification and sequencing of the mitochondrial genome of Paragonimus ohirai.

Primer Sequence (5′->3′) Location
URNLFa AGCCAGGTTGGTTCTTATCTAT rrnL
URNSRa TACCWTGTTACGACTTAHCWCA rrnS
TRECOBFa CAGATGTCYTATTGGGCTGC cob
TRECOBRa GAACHRVCCACARYCCCTTAAAC cob
JB3Fa TTTTTTGGGCATCCTGAGGTTTAT cox1
JB4.5Ra TAAAGAAAGAACATAATGAAAATG cox1
PO1R ACATAGCCACTAACACAGCAC nad2
PO2F TGGTTCGGGTGTTTTCTGCG cob
PO3R CCCACTCAAAACGAAACCTAAG atp6
PO4F CGGTGAGGGTTCCTCTGTTG tRNAAla
PO5F GTCTCACAATCCCAAATCCTGG cox1
PO6F GTTTGGGAAGTGTTGTCTGGG cox1
PO7F TCCTTTTAGGGGAGTAAAGGCC cob
PO8R GCACAACCGCAACAAAGACC atp6
PO9R AGCAGCCCAATCAGAGTTACC rrnS
PO10F CTGCACCAAATCCGAACGCC nad3
PO11F CTTATGGAACTGCAGCACTTC cox2
PO12R CGTACCCAACAAACATAAACC nad5
PO13F AATTTTTCGTGTTAGAGGGAG tRNAGlu
PO14R GAAGATAACGAATTAGCCAAAC cob
PO15F GTGGGTACTTGTATTCTGTG nad6
PO16R GTCAAAACACAAAACAAACAC nad5
PO17R CGCCGTAATTGAAGAACC cox3
PO18F GGTGTAGAAGGGTTGCAC tRNAGlu
PO19F GGCACTTTTTAAGGCTGGTGG nad5
PO20R ACCTCAGATTGGAAGGAAAGCAC NR
PHOR GTTACCAAAGGATCCGCCTGC cob
PHOWR CGGTATGTACCCCAACTAAATC rrnS
POHF ATGATTTGCAGGAGATTTCGGAC rrnS
PHOGLUR CCTCTAACACGAAAAATTAAC tRNAGlu
GLYFa AGTATKYYGTCTTTCCAAGTC tRNAGly
GLYRa ACKAGACCHCYGACTTGGAAAGAC tRNAGly

Notes.

a

Platyhelminth-universal primers used for inital amplification of the corresponding genes/regions to get sequences to design further primers.