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. 2019 Jun 20;7:e7133. doi: 10.7717/peerj.7133

Figure 3. Pairwise variation (V) calculated using GeNorm to determine the optimal number of reference genes.

Figure 3

(TS) different tissues, (SG) developmental stage seedlings, (ST) stress-treated seedlings, and (TT) data from all experimental groups combined. The average pairwise variations Vn/Vn+1 were analyzed between the normalization factors NFn and NFn+1 to indicate the optimal number of reference genes in different samples.