Table 1. Candidate genes, primers, and different parameters derived from the qRT-PCR analysis in H. perforatum.
| Gene symbol | Gene name | Accession No. | Amplicon size (bp) | Primer sequence U/L (5′–3′) | Tm (°C) | Efficiency (%) |
|---|---|---|---|---|---|---|
| ACT2 | Actin 2 | MK054303 | 101 | Fw: AGGAGTCCCTCCACGACCAC | 83.6 | 97.6 |
| Rv: GCCGTTGTGTACCGGGTAGG | ||||||
| ACT3 | Actin 3 | MK106364 | 139 | Fw: ATCCTTCCCACGGTGGTTGC | 88.2 | 102.9 |
| Rv: CAATCGCCTCGTCGCCTACA | ||||||
| ACT7 | Actin 7 | MK106365 | 131 | Fw: GAGCAGCAGCAGGTCGACAA | 83.0 | 107.6 |
| Rv: ACCCACTCGAGCCCAGTGTA | ||||||
| CYP1 | Cyclophilin | MK106359 | 163 | Fw: AGGGATCCAGCTTCCACCGT | 87.8 | 96.2 |
| Rv: GCGTTGGCCATGGAGAGGAT | ||||||
| EF1-α | Elongation factor 1-alpha | MK106356 | 121 | Fw: TGGAGGCTCTCCCTGGTGAC | 85.8 | 105.6 |
| Rv: AAGTTGGCAGCCTCCTTGGC | ||||||
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase A subunit | EU301783 | 76 | Fw: AGGCCTCCCACCTCCTCAAG | 84.7 | 105.1 |
| Rv: GGTTGACAGGGTTGCGGTCA | ||||||
| TUB-α | Alpha tubulin | MK106362 | 132 | Fw: TGCTGCGGTTGCCACCATTA | 84.8 | 109.5 |
| Rv: CGCTGCACCTTTGCAAGATCG | ||||||
| TUB-β | Beta tubulin | MK106361 | 170 | Fw: CGACGGGAGTGACAGCCTTG | 86.5 | 108.6 |
| Rv: CGACCACATCGCTCGTCTCC | ||||||
| UBC2 | Ubiquitin-conjugating enzyme 2 | MK106357 | 103 | Fw: AGGAGGAGGCGCCTTTGAGA | 84.5 | 98.2 |
| Rv: CGCAAGAGCCGGTCCATTCA | ||||||
| GSA | Glutamate-1-semialdehyde 2,1-aminomutase | KJ624985 | 78 | Fw: GGTGTCAGGATGGCGGTGTC | 85 | 94.4 |
| Rv: GGGCTGGCCACCAACTGATT | ||||||
| PKS1 | Polyketide synthase 1 | EF186675 | 76 | Fw: ACGGACGCTGCCATCAA | 82.5 | 98.3 |
| Rv:ACATAACCGTGTACCTTGTC | ||||||
| PP2A | Protein phosphatase 2A | MK106360 | 93 | Fw: GGCAAGTGCCCAGACACCAA | 81.2 | 95.5 |
| Rv: CAGCGCCACTAGCAGCGTAA | ||||||
| RPL13 | Ribosomal protein L | MK106363 | 151 | Fw: CAGCGCTGGATGTTCGAGGA | 84.5 | 92.5 |
| Rv: TGGTGGAAGCCAACCTCCCA | ||||||
| SAND | SAND family protein | MK106358 | 111 | Fw: CTCCCTCGCACTGGGACAAC | 83.2 | 97.1 |
| Rv: AAGACCAGCAGGACCACCCA |