Table 2. Ranking and expression stability values of the candidate reference genes calculated by NormFinder.
Rank | TS | SG | ST | TT | ||||
---|---|---|---|---|---|---|---|---|
Gene | Stability value | Gene | Stability value | Gene | Stability value | Gene | Stability value | |
1 | EF1-α | 0.090 | ACT2 | 0.048 | ACT2 | 0.141 | TUB-β | 0.246 |
2 | TUB-β | 0.248 | TUB-β | 0.048 | ACT7 | 0.179 | ACT2 | 0.332 |
3 | ACT2 | 0.323 | TUB-α | 0.190 | EF1-α | 0.194 | CYP1 | 0.346 |
4 | CYP1 | 0.371 | EF1-α | 0.205 | TUB | 0.195 | EF1-α | 0.398 |
5 | ACT7 | 0.462 | CYP1 | 0.251 | CYP1 | 0.205 | TUB-α | 0.445 |
6 | UBC2 | 0.521 | GSA | 0.304 | TUA | 0.247 | SAND | 0.453 |
7 | GSA | 0.620 | GAPDH | 0.308 | GSA | 0.262 | ACT7 | 0.511 |
8 | PP2A | 0.644 | PP2A | 0.325 | PKS1 | 0.311 | UBC2 | 0.532 |
9 | GAPDH | 0.653 | SAND | 0.337 | SAND | 0.335 | PKS1 | 0.562 |
10 | PKS1 | 0.663 | UBC2 | 0.487 | UBC2 | 0.341 | PP2A | 0.586 |
11 | SAND | 0.687 | RPL13 | 0.491 | RPL13 | 0.353 | RPL13 | 0.594 |
12 | TUB-α | 0.710 | ACT7 | 0.540 | GAPDH | 0.375 | GSA | 0.602 |
13 | RPL13 | 0.789 | PKS1 | 0.771 | PP2A | 0.401 | GAPDH | 0.668 |
14 | ACT3 | 0.934 | ACT3 | 1.297 | ACT3 | 0.462 | ACT3 | 0.688 |
Note:
(TS) Different tissues, (SG) developmental stage seedlings, (ST) stress-treated seedlings, and (TT) data from all experimental conditions combined.