Table 4:
Effect of beads, implanted into the site of injury, on ectopic limb patterning using the ALM treated with Retinoic Acid
| Bead size | Total ALMs performed |
ALMs Counted* |
No Element |
Single Nodule |
Multiple symmetrical Elements |
Complete Limb** |
2 limbs | More than 2 limbs*** |
|---|---|---|---|---|---|---|---|---|
| no bead | 16 | 13 | 0 | 2 (15.4%) | 0 (0%) | 1 (7.7%) | 10 (77%) | 0 |
| 0.5mm beads | 16 | 16 | 0 | 3 (18.8%) | 1 (6.3%) | 9 (56.3%) | 1 (6.3%) | 2 (12.5%) |
| 1 mm beads | 16 | 13 | 0 | 0 | 3 (21.4%) | 7 (58%) | 2 (16.6%) | 0 |
*
Two animals did not survive RA treatment. Three limbs did not have blastemas at time of RA treatment. Percentage is the % of counted ALMs with phenotype.
**
includes phenotypes exhibiting bifurcation in the autopod and/or zeugopod
***
Includes phenotypes with 3 limb axes with one or more axes being distally hypomorphic