FIG. 4.

Neotissues in reconstructed pathological bladders display mucosal and smooth muscle compartments with elevated collagen content. (A–C) Gross and magnified photomicrographs of porcine bladder cross-sections stained with MTS from NSCs, acutely injured groups (post-pBOO) subjected to urinary outlet resistance pressures of 35 ± 10 cmH₂O (m-pBOO) or 70 ± 15 cmH₂O (s-pBOO), and regenerated (RT) and NAC tissues 3 months after bladder reconstruction with BLSF grafts. For (A–C), scale bars for first columns are 5 mm, second columns are 2 mm, and third to fourth columns are 200 μm. (D) Quantitation of collagen content in MTS-stained specimens described in (A–C). For all panels, N = 3–10 animals per group were assessed per data point. *p < 0.05 in comparison to NSC. ^†p > 0.05 in comparison to RT. Data were analyzed with one-way ANOVA and post hoc Student's two-tailed t-test. Means ± SD. MTS, Masson's trichrome; NAC, nonaugmented control; NSCs, nonsurgical controls.