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. 2018 Nov 5;38(1):e99506. doi: 10.15252/embj.201899506

Figure 1. Loss of Vgll4 in tumor cells inhibits tumor growth in syngeneic mouse tumor models.

Figure 1

  1. VGLL4 protein expression levels in six murine tumor cell lines. Total cell lysates were analyzed by Western blot.
  2. VGLL4 protein levels were reduced in Vgll4‐depleted cells. Total cell lysates of Vgll4‐knockdown LLC and MB49 cells were analyzed by Western blot.
  3. Control or shVgll4 LLC cells were transplanted into C57BL/6 mice, and tumor‐growth kinetics was measured at the indicated times. n = 12 tumors for each group.
  4. Quantification of tumor weights 21 days after transplantation of control or shVgll4 LLC cells into C57BL/6 mice from (C). n = 12 tumors for each group. ***P < 0.001, two‐tailed Student's t‐test. The solid line represents the average weight ± SEM.
  5. Control or shVgll4 LLC cells were transplanted into nude mice, and tumor‐growth kinetics was measured at the indicated times. n = 8 tumors for each group.
  6. Quantification of tumor weights 15 days after transplantation of control or shVgll4 LLC cells into nude mice from (E). n = 8 tumors for each group. n.s. P > 0.05, two‐tailed Student's t‐test. The solid line represents the average weight ± SEM.
  7. Control or shVgll4 MB49 cells were transplanted into C57BL/6 mice, and tumor‐growth kinetics was measured at the indicated times. n = 9 tumors for each group.
  8. Quantification of tumor weights 30 days after transplantation of control or shVgll4 MB49 cells into C57BL/6 mice. n = 9 tumors for each group. ***P < 0.001, two‐tailed Student's t‐test. The solid line represents the average weight ± SEM.
  9. Control or shVgll4 MB49 cells were transplanted into nude mice, and tumor‐growth kinetics was measured at the indicated times. n = 8 tumors for each group.
  10. Quantification of tumor weights 15 days after transplantation of control or shVgll4 MB49 cells into nude mice from (I). n = 8 tumors for each group. n.s. P > 0.05; two‐tailed Student's t‐test. The solid line represents the average weight ± SEM.
  11. Immunofluorescent staining of CD3, CD8, and CD45 (red) counterstained with DAPI for DNA (blue) using control or shVgll4 LLC tumors from C57BL/6 mice. Statistical analysis of the percentage of CD3‐, CD8‐, and CD45‐positive cells in the tumors is shown in the right panel, respectively. n = 3 tumors for each group. ***P < 0.001, two‐tailed Student's t‐test, mean ± SEM. Scale bar 100 μm.
  12. Quantification of tumor weights 30 days after transplantation of shVgll4 MB49 cells into C57BL/6 mice receiving anti‐CD4 alone, anti‐CD8 alone, or both anti‐CD4 and anti‐CD8 blocking antibodies. n = 20 tumors for each group. The solid line represents the average weight ± SEM.

Source data are available online for this figure.