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. 2018 Nov 5;38(1):e99506. doi: 10.15252/embj.201899506

Figure EV3. VGLL4‐HF4A rescues the defects of VGLL4‐knockdown tumor cells.

Figure EV3

  1. VGLL4 suppresses A549 cell growth in vitro. The proliferation of A549 cells with VGLL4 or VGLL4‐HF4A expression was detected by CCK8 assay. n = 3, mean ± SEM, **P < 0.01, one‐way ANOVA followed by Dunnett's test.
  2. Overexpression of VGLL4‐HF4A promotes B16F10 tumor growth in C57BL/6 mice. Control or VGLL4‐HF4A‐overexpressing B16F10 cells were transplanted into nude mice (n = 8 tumors each group) or C57BL/6 (n = 11 tumors each group) mice, and tumor volumes were measured 21 days after tumor cell inoculation. *P < 0.05, two‐tailed Student's t‐test. The solid line represents the average weight ± SEM.
  3. Expression of VGLL4‐HF4A attenuates the T cell‐mediated tumor cell killing. Activated T cells and A549 cells were co‐cultured in 24‐well plates for 4 days and surviving cells stained with crystal violet.