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. 2019 Apr 16;26(3):243–260. doi: 10.1093/dnares/dsz005

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© The Author(s) 2019. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Distribution of mapped sequence reads of two different alleles in the E3 gene region. (A) Top: Read coverage of Williams 82 with the default parameters of TMAP. Bottom: Read coverage of PGC010 with the default and optimized parameters of TMAP. The E3 gene of PGC010 has a large deletion in the fourth exon. (B) APKM of amplicons from variant (e3T) and wild type (WT) on the fourth exon of E3. Average APKM of four amplicons (left most) and APKM of each amplicon—(a) AMPL1040313, (b) AMPL1040314, (c) AMPL1037722, and (d) AMPL1036854.