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. 2019 Jan 15;30(2):219–227. doi: 10.1091/mbc.E17-10-0582

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© 2019 Stormo and Fox. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 2: — Chromatids are held together by the canonical cohesin complex in endocycled cells. Representative wing imaginal disc cell (A–D) and papillar cell (E, F) chromosome spreads. Corresponding diagrams depict cohesin state. 4’6-Diamidino-2-phenylindole (DAPI) marks DNA. Ploidy is indicated (N value, top right). (A) Heat-shocked wild-type (w¹¹¹⁸). (B) Cells in which rad21^TEV is the only source of Rad21. (C) HS>fzr plus HS>TEV protease. (D) HS>fzr plus HS>TEV protease in a rad21^TEV background. (E) Wild-type (byn>gal4 alone). (F) byn>gal4 driving upstream activating sequence (UAS)>TEV in a rad21^TEV background. Scale bar = 5 µm (main images), 1 µm (insets). (G) Quantification of metaphase spreads in A–D. From left to right, N = 77, 11, 34, and 59 cells per genotype. (H) Quantification of metaphase spreads in E and F. From left to right, N = 22, 52 cells pergenotype.