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. 2019 Feb 1;30(3):357–369. doi: 10.1091/mbc.E18-04-0244

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© 2019 Gujrati et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 6: — SUMOylation deficient PPL is sensitive to phosphatase-inhibitor-induced IF collapse. (A) Time-lapse images at indicated time point for a representative HeLa cell expressing GFP-tagged PPL-C treated with okadaic acid. Scale bars = 10 μm. (B) Time-lapse images at indicated time point for a representative HeLa cell expressing GFP-tagged PPL-C K1646R mutant treated with okadaic acid. Scale bars = 10 μm. (C) Quantification of okadaic acid treated cells (n = 48 cells from N = 5 independent experiments) highlighting onset of granule formation. Asterisks indicate statistical significance p < 0.01. (D) Quantification of okadaic acid treated cells (n = 48 cells from N = 5 independent experiments) at different time points indicating percentage of cells having soluble granules. (E) GFP-tagged PPL-C or PPL-C K1646R mutant transfected cells were treated with OA or left untreated for the indicated time points. Total detergent-soluble (S) and insoluble (P) cell fractions obtained from cells were immunoblotted with GFP antibodies.