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. 2019 Feb 1;30(3):387–399. doi: 10.1091/mbc.E18-09-0548

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© 2019 Melville et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 2: — FABP5 enhances lipoprotein secretion. (A) Immunoblot of top fraction of OptiPrep gradient from budding reactions with McArdle donor membrane. (B) Immunoblot of budding reaction for dose response to FABP5. (C) Immunoblot of APOB in wild-type McArdle cells (WT), McArdle transformed by lentivirus to overexpress FABP5 (tgFabp5) or CRISPR/CAS9-mediated knockout cells (Fabp5^−/−). β-Actin used as a loading control. Brefeldin A (BFA) added to inhibit secretion and show baseline levels of cellular APOB. (D) APOB secreted into the medium of those cells after 3 h and Ponceau-S stain of BSA in membrane for loading control. (E) Quantification of collagen secreted into media (n = 3).