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. 2019 Feb 1;30(3):387–399. doi: 10.1091/mbc.E18-09-0548

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© 2019 Melville et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 3: — FABP5 leads to a subpopulation of cargoes of increased size. (A) Optiprep flotation scheme to separate high lipid VLDL particles from small budded cargo vesicles. (B) Immunoblot showing which fractions correspond to which vesicular cargo. (C) Negative-stain EM of fractions 1 and 3 from budding reactions using McArdle donor membranes, GTP, COPII, and FABP5. Scale bar: 200 nm. (D) Concentration of different size particles from budding reactions using McArdle and (E) HeLa donor membranes (n = 3) measured by Brownian motion with a NanoSight particle analyzer. (F) Relative size of FABP5-positive particles represented by side scatter of particles in flow cytometry with a LSR Fortessa with output from budding reactions monitored using fluorescently labeled FABP5 and SAR1.