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. 2019 Feb 1;30(3):387–399. doi: 10.1091/mbc.E18-09-0548

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© 2019 Melville et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 5: — FABP5 alters the GTPase cycle of SAR1. (A) Tryptophan fluorescence assay measuring loading of GTP into SAR1 in the presence of FABP5 and PREP. (B) Concentration of particles at specific sizes generated from GUVs incubated with SAR1 and FABP5. (C) Tryptophan fluorescence assay measuring GTPase activity of SAR1 in the presence of indicated COPII components. (D) Quantification of the slope of linear regression curves fitted by the least-squares method for GTPase activity from 100 to 200 s (n = 3). (E) Tryptophan fluorescence assay measuring GTPase activity of SAR1 in the presence of indicated tagged COPII components in rapamycin-induced FRB/FKBP12 binding reaction.