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. 2019 Feb 1;30(3):400–410. doi: 10.1091/mbc.E18-05-0297

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© 2019 Sullivan et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 1: — EGOC regulates TORC1-body formation. (A) Kog1-YFP localization before (log growth) and 60 min after glucose starvation in the wild-type strain, and strains missing Gtr1/2 (gtr1Δgtr2Δ), Npr2 (npr2Δ), or carrying a constitutively active GTR1^Q65L allele (Gtr1^on). The dashed lines show the position of each cell in the bright-field image. (B) Time-course data showing the fraction of cells containing Kog1-YFP puncta in the wild-type strain and strains missing Npr2, Gtr1, Gtr2, Gtr1/2, Ego1, Ego3, or carrying a constitutively active Gtr1 allele (Gtr1^on), during glucose starvation (as labeled). Each time point shows the average and SD from experiments carried out on three different days, with 75–300 cells per time point per replicate. The solid lines show the best fit to a single exponential for the gtr1Δgtr2Δ, ego1Δ, ego3Δ, gtr1Δ, and gtr2Δ strains, a double exponential for the wild-type strain, and a straight line for the npr2Δ and Gtr1^on strains. The broken lines in the bottom panels show the best fit to the wild-type data (from the top panel) for comparison.