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. 2019 Feb 1;30(3):400–410. doi: 10.1091/mbc.E18-05-0297

FIGURE 4:

FIGURE 4:

Cooperation between Gtr1 and key regulators of TORC1-body formation. Impact that deleting key regulators of TORC1-body formation, or mutating the prion domains in Kog1 (PrDm1+2), has on Kog1-YFP puncta formation in a gtr1Δ background. Black circles show the percentage of cells with bodies in log growth cultures, while black squares show the percentage of cells with bodies after 60 min of glucose starvation. The values shown are the average from experiments carried out on at least two different days with >100 cells per time point, per replicate. The SD is <5% for all mutants and time points except for sak1Δ (60 min), gcn2Δ (60 min),  pib2Δ (60 min), cka1Δ (0 min), and sit4Δ (0 min), which have standard deviations of 5–10%; npr2Δ (0 min),  sit4Δ (0 min), and sit4Δ (60 min), which have standard deviations of 10–15%; and slt2Δ (60 min) and npr2Δ (60 min), which have standard deviations of 15–20%. The red circle and square on the x-axis shows the data for the gtr1Δ single mutant for comparison.