Fig. 4.

C-C chemokine receptor type 5 (CCR5) is required for poly(inosinic:cytidylic) acid [poly(I:C)]-stimulated inflammatory gene expression by mouse macrophages. A: peritoneal exudate cells, isolated from wild-type or CCR5^−/− mice, were treated for 15 or 30 min with poly(I:C) (50 µg/ml), and ERK, JNK, and p38 phosphorylation (p-) was examined. p38 levels are shown as loading control. B: after 4 h of treatment with poly(I:C) + IFN-γ, total mRNA was harvested, and inducible nitric oxide synthase (iNOS), pro-IL-1β, and cyclooxygenase (COX)-2 mRNA accumulation in wild-type and CCR5^−/− macrophages was quantified by real-time PCR. mRNA accumulation was normalized to GAPDH mRNA levels. C and D: expression of iNOS, COX-2, and pro-IL-1β, as determined by Western blot analysis, and nitrite production was examined in wild-type and CCR5^−/− macrophages treated for 24 h with or without poly(I:C) + IFN-γ. GAPDH levels are shown as loading control. Results are representative (A and C) or means ± SE (B and D) of 3 independent experiments. *P < 0.05.