Fig. 6.

Effects of antibody neutralization of C-C chemokine receptor type 5 (CCR5) on macrophage release of type I IFNs. A–C: RAW264.7 macrophages were pretreated with varying concentrations of neutralizing goat anti-CCR5 or control IgG antibody for 4 h before addition of poly(inosinic:cytidylic) acid [poly(I:C)]. After 24 h of incubation, nitrite formation was determined by Griess assay, and release of IFN-α and IFN-β was evaluated by ELISA. Results are means ± SE of 3 independent experiments. *P < 0.05.