Fig. 5.

Natural killer (NK) cell depleted (NKD) reduced uterine perfusion pressure (RUPP) serum attenuates mitochondrial reactive oxygen species (mtROS) production in endothelial cells. Human umbilical vein endothelial cells (HUVECs) treated with 10% RUPP serum (n = 3) show a significant increase in mtROS vs. cells treated with normal pregnant (NP) serum (n = 4, P = 0.0093). Furthermore, RUPP+NKD serum (n = 7) attenuated mtROS in HUVECs vs. RUPP serum (P = 0.0225). Data are presented as means ± SE. Student’s t-test was used to perform to statistical analysis between 2 respective groups. *Significance compare with NP; #significant compared with RUPP.