Skip to main content
. 2019 Mar 20;316(5):R678–R686. doi: 10.1152/ajpregu.00388.2018

Fig. 4.

Fig. 4.

Chronic intermittent hypoxia (CIH)-exposed mice have reduced tumor burden but more bone marrow tumor cell engraftment. A: representative flow cytometry showing green fluorescent protein (GFP)+ 5TGM1 cells in bone marrow and spleen of a paralyzed KalwRij mouse, which serves as a positive control for 5TGM1 engraftment. B: representative flow cytometry showing GFP+ 5TGM1 multiple myeloma (MM) cells in bone marrow and spleen of a paralyzed C57BL/6J mouse exposed to CIH, showing decreased tumor burden. C: tumor cell burden was higher in bone marrow and spleen of KalwRij mice compared with paralyzed, CIH-exposed mice. D: ratio of bone marrow to spleen 5TGM1 cells is ≅1 in KaLwRij mice, indicating equal distributions between these tissues. In paralyzed CIH-exposed mice, 5TGM1 cells preferentially engrafted in bone marrow. E: 5TGM1-specific monoclonal immunoglobulin G (IgG2b) levels were elevated in paralyzed CIH mice compared with reference levels from normal nonimmunized C57BL/6 controls (dotted lines and gray shading represent upper and lower confidence intervals 8 healthy, 8-wk-old, nonimmunized. C57BL/6J mice). Levels of IgG2b in paralyzed CIH mice were significantly lower than in positive control KalwRij mice. **P < 0.05, ***P < 0.01.