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. 2019 Jun 4;116(25):12327–12336. doi: 10.1073/pnas.1820928116

Fig. 5.

Fig. 5.

Ectopic ventral RAB-3 puncta in let-7(n2853ts) mutants colocalize with postsynaptic muscle acetylcholine receptors (UNC-29::RFP). (A) A diagram showing the timing of expression of let-7 miRNA and its targets lin-41 and hbl-1 during larval development. (B) Wild-type animals expressing GFP::RAB-3 in DA9 motor neurons (wyIs85) at 5-d adult stage (B1). let-7(n2853ts) mutants show ectopic dendritic localization of GFP::RAB-3 puncta in 5-d-old adult animals (B2). Schematic diagrams showing phenotypes are displayed below each image. Green dots, GFP::RAB-3; D, dorsal processes; V, ventral processes. (Scale bars, 10 μm.) (C) Quantification of ectopic dendritic RAB-3 puncta localization phenotype. n = 36 worms in each genotype. *P < 0.05, Student’s t test. Error bars are SEM. (D) Colocalization of DA9 presynaptic terminals (GFP::RAB-3) with postsynaptic muscle acetylcholine receptors (UNC-29::RFP). Wild-type or let-7(n2853ts) mutant animals coexpressing GFP::RAB-3 and UNC-29::RFP were imaged at 5-d-old adult stage. (Scale bars, 10 μm; scale bars apply to Right panels as well.) Arrows show the colocalization of presynaptic and postsynaptic terminals ectopically.