Fig. 4.

ANO1 is functional in embryonic brain slices. (A) A captured image of a cultured RGC in an E14.5 embryonic brain slice in which whole-cell currents were recorded. The recording glass electrode is marked by dashed lines. (B) Representative traces of Ca²⁺–induced Cl⁻ currents in RGCs in embryonic brain slices with or without 100 μM MONNA, an ANO1-specific blocker. To record Ca²⁺-activated Cl⁻ currents, the pipette contained 1 μM Ca²⁺ in 140 mM CsCl solution. Oxygenated artificial cerebrospinal fluid was used for the bath solution; E_hold = −70 mV. (C) Summary of the amplitudes of Ca²⁺-activated Cl⁻ currents in RGCs in embryonic brain slices with or without MONNA.