Fig. 1.
UBE3A negatively regulates PP2A activity, as well as the protein level of its activator PTPA. (A) PP2A activity from brain tissues in WT, Ube3am−/p+ at P7 (n = 3); P17–P20 (n = 3); P25–P28 (n = 4); and P30–P37 (n = 7) (n represents the number of mice). (B) Quantitative mass spectrometry analysis of the protein levels of PP2A-related proteins in the cerebellum and hippocampus based on SILAM. Black dots, proteins with no changes in levels; red dots, PTPA. (C) PTPA protein level was increased in the cerebellum of Ube3am−/p+ mice at P30. n = 8 mice per condition. In all quantifications, error bars indicated mean ± SEM; *P < 0.05, Student’s unpaired t test.