Fig. 3.

PTPA is required for UBE3A-mediated spine maturation. (A) Representative images of dendritic spine of Golgi stained Purkinje cells from WT, Ube3a^m−/p+, Ube3a^m−/p+;Ptpa^+/−, and Ptpa^+/− mice. m, mushroom; s, stubby; t, thin. (Scale bar, 2 μm.) (B) Quantitation of dendritic spine subtypes. n = 60–80 dendrites per condition, n = 3 mice per genotype. (C) Representative images of apical dendrites of hippocampal CA1 pyramidal neurons in WT, Ube3a^m−/p+, Ube3a^m−/p+;Ptpa^+/−, and Ptpa^+/− mice. (Scale bar, 2 μm.) (D) Quantitation of dendritic spine subtypes. n = 20–40 dendrites per condition, n = 3 mice per genotype. (E) Representative images of basal dendrites of layer 2/3 pyramidal neurons of the motor cortex in WT, Ube3a^m−/p+, Ube3a^m−/p+;Ptpa^+/−, and Ptpa^+/− mice. (Scale bar, 2 μm.) (F) Quantitation of dendritic spine subtypes of motor cortex. n = 30–40 dendrites per condition, n = 3 mice per genotype. In all quantifications, error bars indicate mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001, two-way ANOVA with Bonferroni posttest.