Fig. 1.

Ser/Thr phospho-peptide profiling of thymocytes and generation of PP2Ac conditional knockout mice. (A) Ser/Thr phosphorylation measured by MS-based iTRAQ phosphor-peptide analysis in C57BL/6 resting and activated thymocytes. About 350 proteins changed their phosphorylation (log₂). Proteins in which phosphorylation was notably changed are annotated such as Rab12 (green font) and Prpf4b (red font). (B) Ser/Thr phosphorylation measured by MS-based iTRAQ phosphor-peptide analysis in PP2Ac cKO resting and activated thymocytes. Proteins which cannot be found but which notably changed in wild-type cells (A) are annotated, for example Rab12 Prpf4b. The dephosphorylation of some proteins was reduced such as Smarca4. (C) Heatmap showing the 35 proteins showing the greatest change in phosphorylation as measured by mass spectrometry (mean change 1.75-fold). Six of them are related to cell survival (marked with red font). (D) Pathway analysis of the 35 proteins showed the greatest change in phosphorylation in CD3-stimulated PP2A-deficient thymocytes.