Fig. 3.

Phenotype of peripheral splenic T cells in PP2Ac cKO mice. (A) Cell surface staining of CD4 and CD8 on WT and PP2Ac cKO splenocytes, Bar charts indicate frequency and cell number of splenocyte subpopulations. (B) Expression of CD62L and CD44 on CD4⁺ splenocytes from WT and cKO mice; bar charts show frequency of CD4⁺ cells in the CD62L^hiCD44^lo and CD62L^loCD44^hi subpopulations. Data are representative of five experiments. (C and D) Flow cytometry of splenocytes from WT or cKO mice expressing a transgene encoding the MHC class II-restricted OT-II (C) and OT-I (D) TCR. *P < 0.05; **P < 0.01; ***P < 0.001.