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. 2019 May 31;116(25):12422–12427. doi: 10.1073/pnas.1821116116

Fig. 5.

Fig. 5.

Bone marrow-chimera experiments. (A) Flow cytometry of thymus cells from bone marrow chimeras deficient in recombination-activating gene 1 (Rag1−/−) 8 wk after transfer of bone marrow cells from WT C57BL/6 (CD45.1+) mice and WT or PP2A cKO(CD45.2+) mice, assessed for expression of CD4 and CD8 after gating on CD45.1 or CD45.2. Data are representative of three experiments. (B) Apoptosis of C57BL/6 (CD45.1+) and WT (CD45.2+) total thymocytes in reconstituted Rag1−/− mice. (C) The apoptosis of DN and DP cells by annexin V staining before and after anti-CD3 stimulation in WT or PP2A cKO(CD45.2+) derived cells. (D) BrdU incorporation in WT or PP2A cKO(CD45.2+) mice. Data are representative of three experiments. *P < 0.05 and **P < 0.01.