Fig. 6.

Bcl2 transgenic mice can rescue cell apoptosis. (A) Cell surface staining of CD4 and CD8 on WT and cKO on Bcl2⁻ or Bcl2⁺ background thymocytes. Numbers in or adjacent to outlined areas (or in quadrants) indicate percent. Bar charts show the frequency and cell number of total, DN, DP, CD4⁺, or CD8⁺ SP thymocyte subpopulations. (B) Cell surface staining of CD4 and CD8 on WT and cKO on Bcl2⁻ or Bcl2⁺ background splenocytes, and cell number of splenocyte subpopulations. (C) Cell staining of annexin V of WT, cKO, WT Bcl2⁺, and cKO Bcl2⁺ DN and DP thymocytes with or without anti-CD3 stimulation, and quantification of annexin V cell percentage. Data are representative of five experiments. *P < 0.05; **P < 0.01; ***P < 0.001.