Fig. 4.

VEGF-pathway regulates the expression of biomineralization, regulatory and vascularization genes. (A–D) In each panel we present: gene-expression level for control and VEGFR inhibited embryos showing average expression level and individual measurements [measured by RNA-seq in fragments per kilobase of transcript per million mapped reads (FPKM); **P < 0.05 after FDR correction; 16 hpf and 20 hpf, n = 2; 24 hpf and 30 hpf, n = 4)]; temporal expression profile and initiation time (quantitative PCR, n = 3, error bars correspond to SD); spatial gene expression in control and in VEGFR inhibited embryos (whole-mount in situ hybridization, n = 3, lateral view). (E, Left) Illustration of Pl-VEGF expression (blue) and VEGF target gene expression (red). (Right) Examples of VEGF target gene expression in a vegetal view. Image courtesy of Yarden Ben-Tabou de-Leon (artist). (Magnification, 200×.)