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. 2019 May 31;116(25):12353–12362. doi: 10.1073/pnas.1902126116

Fig. 5.

Fig. 5.

VEGF target, Pl-Rhogap24l/2, is essential for normal skeletogenesis. (A–E) Pl-rhogap24l/2 perturbations. (A) Embryo injected with control MO shows normal skeleton at 3dpf. Pl-rhogap24l/2 knockdown using either translation MO (B) or splicing MO (C) results in ectopic branching at the postoral and body rods at 3 dpf. (D) GFP overexpression results in normal skeleton while (E) overexpression of Pl-rhogap24l/2 results in ectopic branching at the postoral and body rods, sometimes within the same embryo. (Scale bar, 50 μm.) (Enlargement magnification, 200×.) (F) Quantification of Pl-rhogap24l/2 perturbations, color code is indicated in the representative images (A–E) (n = 3–5, exact number of embryos scored is provided in SI Appendix, Table S1).