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Effects of TPMs on actin disassembly by Cof1. Oregon Green (OG)-labeled actin filaments (teal) were preassembled in the presence or absence of the indicated TPM isoforms (2 μM). Then, excess actin monomers were flowed out, and the same TPM isoform (2 μM) was flowed in together with Cy3-labeled human Cy3-Cof1 (150 nM). Scale bar, 10 m. Time stamp in min:sec.
