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Effects of TPMs on actin disassembly by Cof1, Cor1B and AIP1. Oregon Green (OG)-labeled actin filaments (teal) were preassembled in the presence or absence of the indicated TPM isoforms (2 μM). Then, excess actin monomers were flowed out, and the same TPM isoform (2 μM) was flowed in together with 150 nM Cy3-Cof1 (yellow), 15 nM Cor1B, and 15 nM AIP1. Scale bar, 10 m. Time stamp in min:sec.
