Figure 2.

PsbP protein binds to geminivirus DNA in vitro. The autoradiograph of electrophoretic mobility shift assay (EMSA) showing purified MBP‐PsbP protein binding to the SCR region probe in the presence of ATP (a, b), the βC1 region probe (c) of betasatellite and the CR region probe (d) of helper geminivirus DNA‐A. Autoradiograph of EMSA showing binding of PsbP protein to single‐stranded DNA (ssDNA) (e), and double‐stranded (dsDNA) (f) fragment of betasatellite genome in the presence of competitor DNA (either NbActin ssDNA or pMAL‐c2X plasmid). Location of probe into the geminivirus and betasatellite DNA is indicated for each autoradiograph. The purified MBP protein was used as negative control.