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. 2019 Apr 15;20(7):943–960. doi: 10.1111/mpp.12804

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© 2019 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Silencing of PsbP accelerates disease development in N. benthamiana. (a) Betasatellite induced symptoms on wild‐type, PsbP‐silenced or pTRV‐infiltrated plants at 17 days post‐inoculation (dpi). (b) Comparative level of PsbP transcripts in PsbP‐silenced or pTRV‐infiltrated N. benthamiana plants detected by northern blotting analysis at 10 days post‐infiltration of silencing constructs. Ethidium bromide stained gels showing ribosomal RNA (rRNA) served as loading control. P1, P2 and P3 indicate samples from three independent plants. (c) Appearance of RaLCB‐mediated symptoms at different dpi on wild‐type, PsbP‐silenced and pTRV‐infiltrated plants. Southern blotting analysis indicating comparative level of DNA‐A (d) and DNA β (e) in either PsbP‐silenced or pTRV‐infiltrated N. benthamiana plants. The ethidium bromide stained gel showing plant genomic DNA (gDNA) served as loading control. P1 and P2 indicate samples from two independent plants. Viral DNA forms are indicated as OC (open circular), SC (supercoiled) and SS (single stranded).