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. 2019 Apr 15;20(7):943–960. doi: 10.1111/mpp.12804

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© 2019 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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PsbP overexpression interferes with symptom induction and viral DNA accumulation during betasatellite infection. (a) PsbP overexpressing (35: PsbP) and wild‐type N. benthamiana plants exhibiting symptom induction following betasatellite infection. (b) Appearance of betasatellite‐mediated symptoms on wild‐type and PsbP overexpressing transgenic N. benthamiana plants. Southern blotting analysis indicating comparative level of DNA‐A (c) and DNA β (d) in wild‐type and PsbP overexpressing transgenic N. benthamiana plants. P1, P2 and P3 indicate samples from three independent plants. Viral DNA forms are indicated as OC (open circular), SC (supercoiled) and SS (single stranded). Ethidium bromide stained gel showing plant genomic DNA (gDNA) serves as loading control.