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. 2019 Apr 15;20(7):943–960. doi: 10.1111/mpp.12804

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© 2019 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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PsbP‐mediated impediment against geminivirus infection depends on overall expression of PsbP isoforms. (a) Betasatellite induced symptoms on wild‐type and transgenic PsbP dRNAi (1Air and 2FAir) N. tabacum plants. (b) Symptom severity graph shows appearance of betasatellite‐mediated symptoms on wild‐type, 1Air and 2Fair N. tabacum plants at different dpi. Southern blotting analysis shows comparative level of DNA β at 14 dpi (c) and 21 dpi (d) in wild‐type, 1Air, and 2FAir N. tabacum plants. P1, P2 and P3 denote samples from three independent plants. Viral DNA forms are indicated as OC (open circular), SC (supercoiled) and SS (single stranded). Ethidium bromide stained gel showing plant genomic DNA (gDNA) serves as loading control.