Table 2.
Equations produced by curve fits using GraphPad Prism 7 and the HI thresholds calculated from the equations
| Analytes | TAE | Functions | R2 | Hemolysis Index threshold | Hemoglobin † (g/L) | |
|---|---|---|---|---|---|---|
| Aspartate aminotransferase | +20% | Bias% = 13.63 + 0.63*HI‐0.29e‐03*HI^2 | 0.98 | P < 0.05 | 10.16 | 0.65 |
| Total bile acid | −10%a | Bias% = 0.65‐0.26*HI+18.53e‐05*HI^2 | 0.98 | P < 0.05 | 42.23 | 0.92 |
| Lactate dehydrogenase | +20% | Bias% = 24.04 + 1.47*HI‐91.81e‐05*HI^2 | 0.98 | P < 0.05 | NA | >0.5 |
| Creatine kinase | +30% | Bias% = −2.03 + 0.41*HI‐0.10e‐02*HI^2 + 1.04e‐06* HI^3 | 0.99 | P < 0.05 | 99.96 | 1.40 |
| Hydroxybutyrate Dehydrogenase | +30% | Bias% = 18.15 + 1.50*HI‐92.35e‐05*HI^2 | 0.99 | P < 0.05 | 7.94 | 0.63 |
| Uric acid | −17% | Bias% = −0.06*HI+5.20e‐05*HI^2 | 0.85 | P < 0.05 | 500 | 4.73 |
| Creatine kinase‐MB |
+3 s (11.58 U/L) |
Bias = −1.11 + 0.29*HI‐21.21e‐05*HI^2 + 1.75e‐007*HI^3 | 0.99 | P < 0.05 | 45.20 | 0.94 |
| Potassium |
+0.5 mmol/L |
Bias = 0.82*log(HI) | 0.90 | P < 0.05 | 4.07 | 0.60 |
| Sodium |
−4 mmol/L |
Bias = −94.42e‐04*HI+0.74 | 0.89 | P < 0.05 | 501.8 | 4.75 |
TAE, total allowed error recommended by CLIA’88 regulations; NA, not available. The total allowed error for hydroxybutyrate dehydrogenase was assumed to be 30% according to experience.
a
±10% bias was set as the accepted TAE for the analytes because they are not included in the CLIA’88 regulations. Hemoglobin was estimated from equation HI = 120*Hb‐68, supplement Figure S1.