Figure 1.

Expression of podoplanin on lymphatic endothelial cells and effect of platelets on their migration. A) Flow cytometer, frequency distributions of fluorescence intensity for LEC and HMEC-1 labeled with antibody against podoplanin (PDPN). B) Effect on migration when different numbers of platelets were added to LEC which had been settled onto a Transwell filter, with or without treatment with VEGF-C (300 ng/ml). The percentage of the LEC which had transmigrated after 24h was analyzed. Data are mean ± SEM from four experiments. ANOVA showed significant effects of VEGF-C and of platelet count (p < 0.01 in each case). **=p < 0.01 compared to 0 platelets by Dunnett’s test, for VEGF-C-treated LEC. C) Effect on migration when platelets (10⁸) were added to HMEC-1 which had been settled onto a Transwell filter, with or without treatment with VEGF-C (300 ng/ml). The percentage of the HMEC-1 which had transmigrated after 24 h was analyzed. Data are mean ± SEM from three experiments. ANOVA showed significant effects of VEGF-C and of platelets (p < 0.01 in each case). **=p < 0.01 compared to 0 platelets by Dunnett’s test, for VEGF-C-treated LEC.