Fig. 1.

SLC38A2 (SNAT2), SLC7A5, and SLC1A1 are the hypoxic-induced AA transporters in breast cancer cell lines. Ten breast cancer cell lines (columns) cultured in normoxia and hypoxia and submitted to RNA-seq were arranged by supervised average-linkage hierarchical clustering. Heatmap colors represent relative mRNA expression of the selected AA transporters (rows), with higher (red) or lower (blue) expression in hypoxia compared with normoxia. AA transporters that mediate the uptake of gluconeogenic (G) or ketogenic (KG) essential (E) or nonessential (NE) AAs that enter specific steps of the TCA cycle (α-ketoglutarate, fumarate, oxaloacetate, and pyruvate) were clustered. The black box represents the cluster composed of three AA transporters (SLC1A1, SLC7A5, and SLC38A2) in eight cell lines. S, semiessential; TRN, triple receptor-negative.