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. 2019 Mar 15;30(6):766–777. doi: 10.1091/mbc.E18-07-0462

FIGURE 4:

FIGURE 4:

GRASP depletion reduces cell migration and invasion. (A) Western blots of WT and GRASP-KO HeLa cells for GRASP55, GRASP65, and actin. (B) GRASP depletion reduces cell migration and invasion. Indicated WT and GRASP-KO HeLa cells were analyzed in a Transwell assay. Images were taken after a 20-h migration. Scale bar: 500 µm. (C) Quantitation of the migration rate in B. Results are presented as mean ± SEM; statistical analysis was performed by comparison with WT cells using Student’s t test. (D) GRASP expression rescues the decreased cell migration in GRASP-KO cells. GRASP55-KO or GRASP65-KO cells transfected with indicated constructs were analyzed by a Transwell assay. Example images are shown in Supplemental Figure 3. Note that defects in cell invasion in GRASP55-KO and GRASP65-KO cells were rescued by the expression of GRASP55-GFP and GRASP65-GFP, respectively, but not by GFP alone. Results are presented as mean ± SEM; statistical analysis was performed by comparison with WT cells (ctrl) using Student’s t test. *, p < 0.05; **, p < 0.01; ***, p < 0.001.