FIGURE 5:

Structural clustering of Ste50-RA domain residues according to their functional phenotypes. Residues affecting the pheromone, hyperosmolar signaling, or both pathways are colored in red, blue, or yellow, respectively, and are represented as atomic spheres (A) and the molecular surface (B) of the NMR structure of the Ste50-RA domain (Ekiel et al., 2009). The pathway-specific cluster residues are located at a distance from each other on opposite sides of the domain structure, and residues affecting both pathways are located between those pathway-specific clusters. (C) Structural bioinformatics metaprediction based on nine different algorithms (see Materials and Methods) showing the propensity of Ste50-RA domain residues to engage in protein–protein interactions. Color-coding of functional residues is as in panels A and B.