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EnaΔLDimer processivity on fascin bundles (corresponds to Figure 3, B, C, and E). Spontaneous assembly of 1.5 μM Mg-ATP-actin (15% Alexa488-actin) with 50 pM MBP-SNAP(549)-EnaΔLΔCC-GCN4 (red) and unlabeled 130 nM human fascin visualized by two-color TIRFM. White arrowheads mark free slow-growing barbed ends, and yellow arrowheads mark fast growing barbed ends associated with EnaΔLDimer. Time interval between frames is 0.5 s.
