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EnaΔL processivity on fascin bundles (corresponds to Figure 1, C, D, I, and K). Spontaneous assembly of 1.5 μM Mg-ATP-actin (15% Oregon green-actin) with 15 pM SNAP(549)-EnaΔL (red) and unlabeled 130 nM human fascin visualized by two-color TIRFM. White arrowheads mark free slow-growing barbed ends, and yellow arrowheads mark fast growing barbed ends associated with EnaΔL. Time interval between frames is 1 s.
