FIGURE 4:

Experimental estimation of the hole size. (A) X-Y views of a finite element calculation of nuclear EGFP-NLS diffusion from a hole in the nucleus (marked by an arrow). The nucleus and cell are modeled as flat cylinders with geometrical parameters taken from experimental measurements in Neelam et al. (2016) and nuclear and cytoplasmic diffusion coefficients from Wu et al. (2009) (see Table 1). (B) Initial rate of EGFP-NLS diffusion from the nucleus into the cytoplasm through the nuclear hole as a function of hole size calculated from the finite element model in A (for details of how the initial rate was calculated from data, see Materials and Methods). (C) Plot shows experimental measurements of initial rate of nuclear EGFP-NLS decrease in the nucleus at different stress levels. Error bars are SEM N ≥ 22 for each stress.